Barnabe-Heider et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods, 2008 Feb;5(2):189-96.
Shibata MA, et al. Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung stasis in a mouse immunocompetent mammary cancer model. Cancer Gene Ther. 2008 Dec;15(12):776-86.
Limura T and Pourquie O. Collinear activation of Hoxb genes during gastrulation is ed to mesoderm cell ingression. Nature, 2006 Aug 3;442(7102):568-71.
Sanada K and Tsai LH. G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors. Cell, 2005 Jul 15;122(1):119-31
Ladher RK et al. FGF8 initiates inner ear induction in chick and mouse. Genes and Development, 2005 Mar 1;19(5):603-13.
NEPA21高效基因转染仪------适用于体外(In Vitro)和活体(In Vivo)类器官转染
让转染更简单、更Free
NEPA GENE公司*研发、生产细胞电转染仪及电融合仪等,其生产CUY21系列电转染仪在研究领域中久负盛名,已被数百篇文献引用,其中不乏高水平杂志的文章,如Nature、Cell、PNAS、Genes & Dvelopment等。
2011年,NEPA GENE推出新一代全能型NEPA21高效基因转染系统。与传统的电转仪相比,NEPA21采用全新设计的电转程序,特别适用于难转染细胞、离体组织或动物活体的转染。配合的电压衰减(Voltage Decay)设计,NEPA21可在获得高转染效率的同时,提高细胞存活率。
主要特点:
全新设计的电转程序,
特别适用于难转染细胞、离体组织或动物活体的转染,
高转染效率、高细胞存活率
电转程序各项参数可见、可调,适用性广
不需要特殊的转染试剂盒辅助,运维成本低,胚胎基因编辑
电转染仪应用范围:
♦ 悬浮转染
适用范围:原代细胞、干细胞、以及各种难转染细胞(如免疫细胞、血液细胞等)
♦ 贴壁转染
适用范围:可以直接对贴壁细胞进行转染,省去了细胞消化、再贴壁的步骤(对提高某些种类细胞的存活率十分重要)!
♦ 离体组织转染
适用范围:组织切片、脑切片、器官、胚胎等
♦ 活体转染
适用范围:大脑、视网膜、肌肉、皮肤、肝脏、肾脏、睾丸等
部分参考文献:
Barnabe-Heider et al. Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nature Methods, 2008 Feb;5(2):189-96.
Shibata MA, et al. Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lungstasis in a mouse immunocompetent mammary cancer model . Cancer Gene Ther. 2008 Dec;15(12):776-86.
Limura T and Pourquie O. Collinear activation of Hoxb genes during gastrulation ised to mesoderm cell ingression. Nature, 2006 Aug 3;442(7102):568-71.
Sanada K and Tsai LH. G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors. Cell, 2005 Jul 15;122(1):119-31
Ladher RK et al. FGF8 initiates inner ear induction in chick and mouse. Genes and Development, 2005 Mar 1;19(5):603-13.